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Figure 1 | Molecular Cancer

Figure 1

From: A yeast-based genomic strategy highlights the cell protein networks altered by FTase inhibitor peptidomimetics

Figure 1

Effect of FTase inhibitor I on growth and Ras2 localization in wt and Δram1 cells. A, BY4741 but not Δram1 cells are sensitive to 10 μM FTase inhibitor I. Growth curves were performed in parallel cultures with or without 10 μM FTase inhibitor I. Optical density (OD600) measurements were taken at the indicated time points. B, GFP-Ras2 localization in FTI-treated BY4741 and Δram1 cells. Total cell extracts were clarified at 3500 × g and fractionated into cytosolic (S15400 × g) and membrane (P15400 × g) fractions as previously described [50]. 30 μg of proteins were separated by SDS-PAGE and immunoblot analysis was performed using α-GFP (upper panels) after Ponceau S staining (lower panels) of nitrocellulose membranes. C, Fluorescence (upper panels) and DIC (lower panels) images of cells used in (B) prior to fractionation.

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