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Figure 7 | Molecular Cancer

Figure 7

From: A yeast-based genomic strategy highlights the cell protein networks altered by FTase inhibitor peptidomimetics

Figure 7

Nuclear morphology in HeLa G1 cells treated with FTI-277. The nuclear morphology of HeLa cells, grown and treated as described in Methods, was analysed throughout the cell cycle by plotting the mean intensity signal in the Hoechst channel (x) over the nuclear area (y) using the ScanR analysis software. G1 cells with an altered nuclear morphology were identified in FTI-treated samples and gated to define the mean intensity and area coordinate of this population. A gallery of images of this population is shown in (A). The same region of untreated (NT) cells is shown in (B). C, the fold change of G1 cells with an altered nuclear morphology in FTI-277 treated versus NT cells present in the gated population was calculated and plotted in the graph; the mean and the standard deviation of two independent experiments are given. D, the upper panel shows the software mask that identifies the nuclei in the images. The lower panel shows galleries of nuclei in FTI-277-treated cells present in the gated population, stained with Hoechst to label nuclei and with anti-Pak (αPaK) and anti-Phospho-histone H3 (αPhoH3) antibody as indicated

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