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Figure 6 | Molecular Cancer

Figure 6

From: TRAIL sensitize MDR cells to MDR-related drugs by down-regulation of P-glycoprotein through inhibition of DNA-PKcs/Akt/GSK-3β pathway and activation of caspases

Figure 6

TRAIL enhanced the cytotoxicity of MDR-related drug in MDR cells by the down-regulation of DNA-PKcs and P-gp via caspase-3 activation. (A) CEM/VLB100 cells were treated with graded single doses of doxorubicin (DOX) or vinblastine (VLB) after pretreatment of low dose of TRAIL (1 ng/ml) for 6 h. The percentage of cell survival after combined treatment of TRAIL with MDR-related drug was determined after 5 days incubation using the MTT assay. Each bar represents the mean ± S.D. of triplicate experiments. ***p < 0.005 versus TRAIL alone treated cells at the same dose point. (B) The cell lysates obtained from CEM/VLB100 cells co-treated with TRAIL (1 ng/ml) and indicated dose of DOX were subjected to western blot analysis to monitor levels of P-gp, DNA-PKcs, caspase-3, PARP, and actin as a loading control.

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Molecular Cancer

ISSN: 1476-4598