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Figure 8 | Molecular Cancer

Figure 8

From: TRAIL sensitize MDR cells to MDR-related drugs by down-regulation of P-glycoprotein through inhibition of DNA-PKcs/Akt/GSK-3β pathway and activation of caspases

Figure 8

Potentiated suppression of DNA-PKcs expression by TRAIL leads to a severe inhibition of both Akt/GSK-3β phosphorylation and P-gp and c-FLIPs expression and enhances the cytotoxicity of TRAIL and of MDR-related drug in MDR cells. (A) CEM/VLB100 cells were transfected with a siRNA against DNA-PKcs or scrambled siRNA as a control. After 48 h, the transfectant was treated with or without TRAIL (1- and 10 ng/ml for 24 h), and the changed level of DNA-PKcs, pAkt (Ser473), tAkt, pGSK3β (Ser9), GSK-3α/β, P-gp, PARP, c-FLIPL/S and actin was performed by western blot analysis. (B) Each transfectant was treated with indicated doses of TRAIL or vincristine (VCR). After 5 days, the percentage of growth inhibition was determined incubation using the MTT assay. Data represent means ± S.D. of triplicate experiments. **p < 0.001, ***p < 0.005 versus cells transfected with scrambled siRNA at the same dose point.

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Molecular Cancer

ISSN: 1476-4598