Skip to main content
Figure 1 | Molecular Cancer

Figure 1

From: Oral cancer overexpressed 1 (ORAOV1) regulates cell cycle and apoptosis in cervical cancer HeLa cells

Figure 1

ORAOV1 silencing inhibits cell growth and colony formation ability in HeLa cells. (A) The expression levels of ORAOV1 mRNA and ORAOV1 protein in HeLa cells upon transfection with 100 nM ORAOV1 siRNA for 48 hrs were analyzed by real time PCR and Western blot assay respectively. 100 nM non-targeting siRNA-transfected HeLa cells and blank cells were used as controls. Results represent the means ± SE (n = 3). *, P < 0.05. (B) The effect of ORAOV1 silencing on HeLa cell growth. (C) Examination the effect of ORAOV1 silencing on HeLa cell proliferation by MTT assay. Results represent the means ± SE (n = 3). *, P < 0.001. (D) Test the effect of ORAOV1 silencing on HeLa cell colony formation ability by plate colony assay. The relative colony formation efficiency of the blank cells was set as 1. Each column represents a mean value of triplicate experiments in each group. Data are Mean ± SE, *, P < 0.001.

Back to article page

Molecular Cancer

ISSN: 1476-4598