Figure 2

ORAOV1 silencing induces S-phase cell cycle arrest in HeLa cells. (A) Quantification of cell cycle distribution determined by flow cytometry analysis at 48 hours posterior to the treatment. Data were Mean ± SE for triplicate experiments. (B) BrdU incorporation assay was performed at 48 hrs posterior to the treatment. Each column represents a mean value of triplicate experiments in each group. Data are Mean ± SE, *, P < 0.05. (C) Western blot analyses of the expression of Cyclin A, Cyclin B1, Cyclin E, Cyclin D1, Cdc2, and Cdk2. G3PDH was used as an internal control for equal loading. Data were representative blots from three independent experiments.