Figure 3

ORAOV1 silencing induces apoptosis in HeLa cells. (A) Analysis of HeLa cell apoptosis (sub-G1 cells) upon ORAOV1 silencing by flow cytometry assay using PI staining. (B) Quantitative analysis of cell apoptosis by Annexin V and PI double staining. Data were Mean ± SE for triplicate experiments, *, P < 0.05. (C) The effect of ORAOV1 silencing on the expression of P53, Bcl-2, Caspase-3, 8 and 9, and cytochrome c (mitochondrial and cytosolic). G3PDH was used as an internal control for protein equal loading, and VDAC and β-actin was used as equal mitochondria and cytosol loading control respectively. Data was representative blots of three independent experiments.