Figure 2

HO-1 target gene expression in 190 human tumors. (A) The heatmap shows the expression pattern of 14 HO-1 target genes including HO-1 itself in the 190 human tumors of the 14 most common cancer types included in the GCM database. Red and blue squares denote high versus low levels of expression of each individual gene normalized to its mean expression across all tumor samples, respectively. (B) Relative gene expression of 8 selected HO-1 target genes. The Y-axis displays the normalized DNA Microarray signal across 190 tumor samples (comprising the X-axis). Cancer types are shown above the plot. (C) mRNA levels were measured by qRT-PCR using the RNA samples isolated from HO-1 expressing (LMP) and HO-1 silenced (miHO-1) BeWo cells. The expression values were normalized relative to Arp. The levels of mRNA in LMP and miHO-1 cells are shown in percentage relative to LMP cells (set to 100%). Bars represent mean (+/- SEM) of three independent experiments.