Figure 4

PXDN and HO-1 co-localize in first trimester placenta. Immunohistochemical analyses of serial sections of placental tissues (between 7 and 14th weeks of gestation) stained with different antibodies, counterstained with DAPI and analysed by fluorescence microscopy as described in Materials and Methods. Trophoblast and stromal cells are marked by Cytokeratin 7 (CK7, green) and Vimentin (VIM, red) staining, respectively. Ki67 (red) staining indicates poorly invasive but proliferative EVT, whereas p57 (green) positive cells account for invasive, differentiated EVT. HO-1 and PXDN were stained on separate serial sections. Photographs were taken at 400-fold magnification. VC, villous core; EVT, extravillous trophoblast; CC, cell column; ST, syncytiotrophoblast. Note the relative higher abundance of PXDN and HO-1 in CTB as compared with EVT. Magnification: 200x.