Upregulation of human MMP13 in response to FCS. A375 cells were starved for two days in presence of 0.5% dialyzed FCS and stimulated by the addition of 100 ng/ml EGF or 10% FCS. After 4 and 8 h, cells were harvested and monitored for the expression of MMP13 by realtime PCR. Human ribosomal S14 was used as reference gene. *: p < 0.05 (Student's t test, paired, two-tailed).