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Figure 3 | Molecular Cancer

Figure 3

From: Cdk5 phosphorylates non-genotoxically overexpressed p53 following inhibition of PP2A to induce cell cycle arrest/apoptosis and inhibits tumor progression

Figure 3

Inhibition of PP2A causes cell-growth inhibition in a p53 dependent manner. (A) HTet23p53, HTet26p53, HTet43GFP and HeLa cells were treated with indicated concentrations of OA with or without Dox for 48 h. Thereafter MTT assay was performed. Bar represents variation within the wells of an experiment (± S.E.). *Represents P < 0.01. (B) Five hundred cells plated were treated as mentioned in (A). After 48 h cells were washed and incubated for 21 days. Cells were stained with crystal-violet and colonies were counted. Bar graph represents average colony number per plate (± S.E.) from three experiments. *Represents P < 0.01. (C) HTet23p53, HTet26p53, HTet43GFP and HeLa cells were transfected with control or p53 siRNA in 96 well-plates, 18 h post-transfection cells were treated with Dox and/or OA and MTT assay was performed after 48 h. Bar graph represents variation within the wells of an experiment (± S.E.). *Represents P < 0.05. HTet26p53 cells were transfected with control or p53 siRNA. Eighteen hour post-transfection cells were treated with Dox and further incubated for 48 h. MTT for cell survival evaluation or western blot analysis detection of p53 was performed.

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