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Figure 4 | Molecular Cancer

Figure 4

From: Cdk5 phosphorylates non-genotoxically overexpressed p53 following inhibition of PP2A to induce cell cycle arrest/apoptosis and inhibits tumor progression

Figure 4

p53 executes cell-growth inhibition by cell cycle arrest and apoptosis. (A) HTet23p53, HTet26p53 and HTet43GFP cells treated with Dox with or without OA and were processed for cell cycle analysis. Bar graph represents average of three independent experiments (± S.E.). *Represents P < 0.05. (B) Cells treated as mentioned in (A) were processed for RT-PCR by utilizing p53, p21, HPV18e6 and β-actin primers. (C) HTet23p53, HTet26p53 and HTet43GFP cells were treated with Dox with or without OA for 48 h and western blot for p53 was performed. β-Actin served as loading control. (D) ChIP assay demonstrates in vivo interaction of overexpressed p53 with the p21 and bax promoters. Cells were treated with Dox with or without OA for 48 h and processed for ChIP assay.

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Molecular Cancer

ISSN: 1476-4598