Figure 4

Topoisomerase I inhibition prevents the association of Myc and Max with the Aurora-A promoter. A. HCT116 were synchronized as described above and analyzed by western blot using antibodies directed against the indicated proteins (n = 3). B. Cells were treated as described above and the mRNAs expressions of Aurora-A, Myc, Max and Mad were evaluated by quantitative RT-PCR (n = 3 +/- sd). C-D. The association of Myc, Max and Mad with the Aurora-A promoter was analyzed in HCT116 by chromatin immunoprecipitation as described above (n = 3 +/- sd). ChIPs were performed using extracts isolated from growing cells (C, lanes 1 and 5, D lane 1), from cells synchronized in the G2 phase of the cell cycle (C, lanes 2 and 7, D lanes 2) or from cells treated by sn38 as indicated.