Figure 5

Topoisomerase I inhibition induces the association of Miz-1 with the Aurora-A promoter and prevents the binding of transcriptional coactivators. A-B. HCT116 were treated as described above and Miz-1 expression was analyzed by western blot (n = 3) or quantitative RT-PCR (B., n = 3 +/- sd). C. ChIP analysis of Miz-1 binding to the Aurora-A promoter in growing cells (lane 1), cells synchronized in G2 (lane 2) or following sn38 treatment. D. The recruitment on the Aurora-A promoter of CBP, RNA Pol II, HDAC1 and the acetylation of histone H3 were analyzed by ChIP using soluble chromatin prepared from cells synchronized in the G2 phase of the cell cycle (serum 9hr, lanes 1, 3, 5 and 7) or treated with sn38 (lanes 2, 4, 6 and 8, n = 3 +/- sd).