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Figure 6 | Molecular Cancer

Figure 6

From: Regulation of the Aurora-A gene following topoisomerase I inhibition: implication of the Myc transcription Factor

Figure 6

The Aurora-A promoter is located within SAHF foci following sn38 treatment. A, B and C. HCT116 cells were treated with sn38 as described above and H2Ax phosphorylation and H3K9 trimethylation were analyzed by immunofluorescence (A), western blot (B) or FACS (C, n = 3). D. SAHF formation was analyzed in growing cells or following treatment by immunofluorescence using DAPI staining (one experiment representative of three). E. HCT116 were treated as described above and the recruitment on the Aurora-A promoter of HP1γ, the trimethylation of histone H3K9 and the phosphorylation of histone H2Ax were analyzed by ChIP using soluble chromatin prepared from cells treated or with sn38 (n = 3 +/- sd)

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Molecular Cancer

ISSN: 1476-4598