Figure 5

Recruitment/attachment of vascular SMC and TCM-induced newly formed pericytes from 10T1/2 mesenchymal stem cells on HUVEC-generated capillary-like structures on Matrigel. A. The photograph showing the attachment of GFP (pZsGreen1C1, GFP-producing vector) -transfected AOSMC on the newly formed capillaries by HUVEC on 3 D matrigel. B. Differential attachment of Q-dot labeled newly formed pericytes from 10T1/2 cells on the HUVEC generated capillaries by RM or different tumor cells derived conditioned media on 3 D matrigel. C. Number of attached Q-dot-labeled newly created pericytes under different tumor cells-conditioned media on capillary-like structures formed by HUVEC on Matrigel. The data are representative mean ± SEM of ten different capillary-like structures. *p < 0.0001 vs RM; #p < 0.05 vs MDA-MB-CM or PaCa-CM. D. Upper panel shows the capillary formation in different treatment conditions, and lower panel shows the attachment of Q-dot labeled 10T1/2 cells on the newly formed capillaries by HUVEC in different treatment conditions on matrigel. RM, Regular Media, MCF-7-CM, MCF-7 cell-derived condition media and MCF-7-CM + PDGF ab¹, MCF-7-CM neutralized by PDGF antibody. Quantification of attachment of Q-dot-labeled cells using software provided by Nikon fluorescent photo microscope. The represents mean ± SEM of 10 capillaries of each experiment of three different experiments. P-values were determined by unpaired students't-test. *p < 0.001 vs RM; **p <0.001 vs MCF-7-CM.