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Figure 9 | Molecular Cancer

Figure 9

From: Brg-1 mediates the constitutive and fenretinide-induced expression of SPARC in mammary carcinoma cells via its interaction with transcription factor Sp1

Figure 9

Fenretinide-induced transactivation and expression of SPARC depend on Brg-1. 4T1, 168FARN or 67NR cells were respectively transfected with siRNA control (-) or siRNA specific for Brg-1 (+). 24 hrs after transfection, cells were transiently co-transfected again with the luciferase reporter vector pREP4-SP-Luc and pRL-CMV. 6 hrs after the second transfection, cells were cultured for 24 hrs with or without fenretinide (5 μM). Total protein extracts were prepared. (A) The luciferase activities were analyzed by the dual-luciferase reporter assay system. (B) Expression of SPARC and Brg-1 proteins were analyzed using Western blot analysis. β-actin was analyzed as a control. (C) Cells were transfected with Brg-1 siRNA or control siRNA for 24 hrs and then treated with fenretinide for 24 hrs. Total RNA was extracted and expression of SPARC mRNA levels was measured by real-time RT-qPCR. Experiments were conducted in quadruplicate and normalized to GAPDH mRNA level. The relative SPARC mRNA level in control siRNA-treated cells is set as 100% (data shown are mean ± SEM, n = 4, **P < 0.001)

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