Figure 4
From: MiR-145, a new regulator of the DNA Fragmentation Factor-45 (DFF45)-mediated apoptotic network
Regulation of endogenous DFF45 expression by miR-145. DFF45 mRNA levels in LS174T cells transfected with miR-145 mimic did not change significantly, by quantitative RT-PCR (A) or real-time PCR assay (B), at either 24 h or 48 h after transfection. (C) The DFF45 protein expression levels was affected significantly by miR-145. LS174T cells were transfected with the miR-145 mimic or miRNA control. DFF45 protein levels were analyzed by Western blotting at either 24 h or 48 h after transfection. All experiments were performed in triplicate and are shown as the mean ± SD.