CSC markers in colon cancer cells and spheroids/colonospheres. A. Representative photographs showing different colon cancer cells (HT-29, HCT-116 wt & p53-null) in adherent condition and colonospheres formed by the respective cell lines. B. The comparative expression of different CSC markers in parental colon cancer cells (referred to as 'P') and the cells from corresponding spheroids (referred to as 'S'). The numbers represent percent of corresponding control normalized to β-actin.