Upregulation of c-myc enhances colonospheres/spheroid formation. A. Western blot showing changes in c-myc levels in the HCT-116 wt cells following transfections of PCMV6-vector or pCMV6-Neo vector containing human cDNA clone for c-myc gene. B. Colonospheres formation for HCT-116 wt parental cells overexpressing c-myc. Each value represents mean ± SD of 3-4 observations. The numbers represent percent of corresponding control normalized to β-actin. *p < 0.001, compared to the corresponding vector-treated control.