From: The Wnt/β-catenin pathway regulates growth and maintenance of colonospheres
Number of cells/well | Number of wells plated | Number of wells showing colonospheres | ||
---|---|---|---|---|
Colonospheres-derived cells | Parental/Vector transfected | Parental/β-catenin siRNA transfected | ||
1000 | 24 | 24 | 24 | 24 |
100 | 24 | 24 | 24 | 10 |
10 | 24 | 24 | 14 | 2 |
1 | 24 | 14 | 4 | 0 |
Sphere forming frequency (95% CI) | 1/2 (1/3-1/2) | 1/11 (1/17-1/7) | 1/171 (1/287-1/102) | |
P value | << 0.0001 |