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Table 1 Extreme limiting dilution analysis of colonospheres forming frequency of HCT-116 cells, transfected with non-targeted (vector) or β-catenin siRNA and by colonospheres-derived cells

From: The Wnt/β-catenin pathway regulates growth and maintenance of colonospheres

Number of cells/well

Number of wells plated

Number of wells showing colonospheres

  

Colonospheres-derived cells

Parental/Vector transfected

Parental/β-catenin siRNA transfected

1000

24

24

24

24

100

24

24

24

10

10

24

24

14

2

1

24

14

4

0

Sphere forming frequency (95% CI)

1/2 (1/3-1/2)

1/11 (1/17-1/7)

1/171 (1/287-1/102)

P value

<< 0.0001

  1. Data are pooled from three independent experiments for each. CI, confidence interval