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Table 1 Extreme limiting dilution analysis of colonospheres forming frequency of HCT-116 cells, transfected with non-targeted (vector) or β-catenin siRNA and by colonospheres-derived cells

From: The Wnt/β-catenin pathway regulates growth and maintenance of colonospheres

Number of cells/well Number of wells plated Number of wells showing colonospheres
   Colonospheres-derived cells Parental/Vector transfected Parental/β-catenin siRNA transfected
1000 24 24 24 24
100 24 24 24 10
10 24 24 14 2
1 24 14 4 0
Sphere forming frequency (95% CI) 1/2 (1/3-1/2) 1/11 (1/17-1/7) 1/171 (1/287-1/102)
P value << 0.0001
  1. Data are pooled from three independent experiments for each. CI, confidence interval