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Figure 1 | Molecular Cancer

Figure 1

From: MiTF links Erk1/2 kinase and p21CIP1/WAF1 activation after UVC radiation in normal human melanocytes and melanoma cells

Figure 1

MiTF was phosphorylated and degraded after UVC. A, Normal human melanocytes were exposed to 3 mJ/cm2 of UVC and collected at various time points for western blot analysis. NHMs from a white individual (top panel) and a black individual (bottom panel) showed a similar response. B, human melanoma c83-2C cells were subjected to 3 mJ/cm2 of UVC and collected for western blot at the indicated time points. C, c83-2C cells were treated with UVC and fixed at the indicated time and subjected to immunofluorescence detection of MiTF protein. DAPI staining was used to show nucleus. D, dose-dependent phosphorylation of MiTF in c83-2C cells. In western blot, either p84 or α-tubulin was used as a loading control.

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