Figure 2
MiTF was phosphorylated by Erk1/2 kinase after UVC. A, NHM (top panel) or c83-2C cells (bottom panel) were pre-treated with various kinase inhibitors and then exposed to UVR at 3 mJ/cm2, and collected 1 hour post-radiation for western blot analysis. B, UVC-mediated Erk1/2 activation and U0126 effect on Erk1/2 inhibition was examined by western blot. NHM cells were pre-treated with U0126, exposed to UVC and collected for western blot 1 hour post-radiation. C, c83-2C cells (top panel) and Malme-3 M cells (bottom) were pre-treated with U0126 and then subjected to UVR at 3 mJ/cm2, and collected at the indicated time points for western blot analysis. D, NHM were pre-treated with p90 RSK-1 inhibitor SL0101 and subjected to UVC, and collected at the indicated time for western blot analysis.