RNAi screening of Ewing's sarcoma cell lines. Ewing's sarcoma cell lines TC-32, TC-71, SK-ES-1 and RD-ES were transfected with siRNA targeting 572 kinases and assayed for cell viability at 96 hr as described in Material and Methods. Assays were done in duplicate and Z-score values for each siRNA treatment was plotted. Each circle represents data for one siRNA and the two colors represent data from the duplicate runs for the same cell lines. The dotted red line indicates the cut off for the significant Z score (-1.65 SD). The darkened circles correspond to the data for chosen hits STK10 and TNK2.