Phenotypic profiling of kinases. (A) Heat map showing the correlation between each independent RNAi screen for the four Ewing's sarcoma cell lines and the normal fibroblast cell line GM05659; Z-scores were used for the generation of the Heat Map. (B) Dendogram showing the correlation coefficients between all the independent siRNA screening runs; Z-scores were used for calculating the correlation coefficients. (C) Venn Diagram on the left depicts the number of significant siRNA hits for each cell line chosen based on significant Z-scores from both the independent runs for every Ewing's sarcoma cell line. Venn diagram on the right shows the overlap of significant siRNA hits between the normal fibroblast cell line and genes significant across all four Ewing's sarcoma cell lines (D) Heat map of Z-score values for the 17 siRNA hits and a PLK1 siRNA. This Heat map was generated using CIMminer http://discover.nci.nih.gov/.