STK10 and TNK2 knockdown leads to decreased cell viability of Ewing's sarcoma cells. TC-71 cells were transfected with siRNAs against STK10, TNK2 or non-silencing control. Growth was assessed by impedance measurements at 1-hour intervals and cell index was plotted as a function of time. (A) Treatment of TC-71 cells with non-silencing siRNA or two different TNK2 siRNA showed reduced cell growth in cells with TNK2 knockdown (B) Treatment of TC-71 cells with non-silencing siRNA or two different STK10 siRNA showed reduced cell growth in cells with STK10 knockdown. Data is representative of three independent experiments. Confirmation of gene silencing was shown by qRT-PCR for (A inset) STK10 and (B inset) TNK2. Each individual siRNA was reverse transfected into TC-71 cells and incubated at 37°C for 48 hours. RNA was extracted 48 hours later and cDNA was prepared using random primers. qRT-PCR was done using specific TaqMan probes and all the results were normalized to GAPDH expression.