Skip to main content
Figure 2 | Molecular Cancer

Figure 2

From: Nicotine-induced survival signaling in lung cancer cells is dependent on their p53 status while its down-regulation by curcumin is independent

Figure 2

Nicotine activates NF-κB in H1299, but not in A549. (A-B) Nuclear extracts were prepared from the cells treated with indicated concentrations of nicotine for 30 min and NF-κB nuclear translocation was assessed by EMSA. (C) H1299 cells were treated with 10-8 M nicotine for different time intervals and EMSA was done. (D) The cytosolic extracts of the time kinetic study of NF-κB activation were Western blotted against the respective antibodies. (E) The nuclear extract of nicotine-treated H1299 cells were incubated with p50 and p65 antibodies or unlabelled oligo and EMSA was conducted to detect supershift and cold competition respectively. (F) H1299 cells were treated with curcumin and then with nicotine for 30 min and EMSA was done. All blots and EMSAs are representative samples of three independent experiments.

Back to article page

Molecular Cancer

ISSN: 1476-4598