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Figure 5 | Molecular Cancer

Figure 5

From: Nicotine-induced survival signaling in lung cancer cells is dependent on their p53 status while its down-regulation by curcumin is independent

Figure 5

Curcumin downregulates nicotine-induced phosphorylation of MAPKs and nuclear translocation of AP-1. (A-B) The cells were treated with nicotine (10-9-10-2M) for 30 min and whole cell lysates were blotted against respective phospho specific antibodies. (C-D) The cells were treated with curcumin and then with nicotine (10-8M for H1299 and 10-6 M for A549) and whole cell lysates were blotted as in (A-B). (E-F) Nuclear extracts were prepared from the cells treated with nicotine (10-9-10-2M) for 30 min. and AP-1 activation was assessed by EMSA. Super shift and cold competition was done by incubating the nuclear extract with c-jun antibody and unlabelled oligo respectively. (G-H) The cells were treated with curcumin and then with nicotine (10-8M for H1299 and 10-6 M for A549) and EMSA was done using nuclear extracts. All blots and EMSAs are representative samples of three independent experiments.

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Molecular Cancer

ISSN: 1476-4598