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Figure 6 | Molecular Cancer

Figure 6

From: Nicotine-induced survival signaling in lung cancer cells is dependent on their p53 status while its down-regulation by curcumin is independent

Figure 6

Over-expression and silencing of p53 produce reciprocal effect on the activation pattern of NF-κB, MAPKs and AP-1. (A-B) H1299 and A549 cells were transfected with pcDNA3-p53 WT and pcDNA3-p53 DN constructs respectively and the stable clones selected were lysed and Western blotted against p53 antibody. (C-D) H1299-p53 WT and A549-p53 DN cells were treated with nicotine (10-9-10-2M) for 30 min, and EMSA was done using nuclear extracts. (E) A549 cells were treated with different concentrations of pifithrin-α (10-50 μM) for 4 h and the whole cell lyasates prepared were Western blotted against p53 antibody. (F) A549 cells were treated with 40 μM pifithrin-α for 4 h followed by nicotine (10-9-10-2M) for 30 min and EMSA was done. (G-H) H1299-p53 WT and A549-p53 DN cells were treated with nicotine (10-9-10-2M) for 30 min, and EMSA was done. (I-J) H1299 WT-p53 and A549 DN-p53 cells were treated with nicotine (10-9-10-2M) for 30 min and whole cell lysates were Western blotted against respective phospho specific antibodies. All blots and EMSAs are representative samples of three independent experiments.

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Molecular Cancer

ISSN: 1476-4598