Figure 8

Nicotine induces proliferation and NF-κB nuclear translocation in Hop-92, NCI-H522 and p53-silenced A549 cells. (A-B) The cells were treated with nicotine and/or curcumin and cell viability was measured by MTT assay. All error bars indicate standard deviation between three independent experiments. (C-D) Nuclear extracts were prepared from the cells treated with indicated concentrations of nicotine for 30 min and NF-κB nuclear translocation was assessed by EMSA. (E) A549 cells were transfected with indicated concentrations of control-si RNA or p53-si RNA and after 48 h, the whole cell lysates were Western blotted against p53 antibody. (F) A549 cells were transfected with indicated concentrations of control-si RNA or p53-si RNA and after 48 h, treated with indicated concentrations of nicotine for 30 min and NF-κB nuclear translocation was assessed by EMSA. All blots and EMSAs are representative samples of three independent experiments.