Figure 3

RhoH upregulates p21 ^Cip1 and p27 ^Kip1 expression. (A) Quantification of p21^Cip1 and p27^Kip1 mRNA expression levels by quantitative SYBR-green PCR in control cells, RhoH and siRhoH-transduced cells. Data were normalised to mRNA levels detected in control cells compared to GAPDH. Statistical significance was analysed using the 's t-test (mean ± SD, n = 3; *P ≤ 0.05). (B, C) Whole cell lysates of control cells, siRhoH and RhoH expressing cells were separated by 4-20% gradient SDS-PAGE and protein expression was detected by antibodies against p21^Cip1 and p27^Kip1. Protein levels were normalised to β-actin expression (lower panel). Quantification and statistical significance were performed using the 's t-test (mean ± SD, n = 2; *P ≤ 0.05).