Role of microRNA-199a-5p and discoidin domain receptor 1 in human hepatocellular carcinoma invasion

Table 2 Primers and probes for miR-199a-5p, DDR1 and reference genes.

Gene	Primer	Sequence (5'→3')
miR-199a-5p	RT	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGGAACAGGT
	Forward	ACACTCCAGCTGGGCCCAGTGTTCAGACTAC
	Reverse	CTCAACTGGTGTCGTGGAGTCGGCAA
	Probe	(6-FAM) TTCAGTTGAGGAACAGGT (MGB*)
RNU44	Forward	CCTGGATGATGATAGCAAATGC
	Reverse	GGCAATTCAGTTGAGAGTCAGTTAGA
	Probe	(6-FAM) ACTGAACATGAAGGTCTT (MGB*)
HMBS (TaqMan assay)	Forward	ATGTCTGGTAACGGCAATGC
	Reverse	GTACCCACGCGAATCACTCT
	Probe	(6-FAM) CTGCAACGGCGGAAGAAAACAGC
DDR1	Forward	AATCGCAGACTTTGGCATGAG
	Reverse	CGTGAACTTCCCCATGAGGAT
GAPDH	Forward	TGCACCACCAACTGCTTAGC
	Reverse	GGCATGGACTGTGGTCATGAG
HMBS (SYBR Green assay)	Forward	TGCAACGGCGGAAGAAAA
	Reverse	ACGAGGCTTTCAATGTTGCC
HPRT1	Forward	TGACACTGGCAAAACAATGCA
	Reverse	GGTCCTTTTCACCAGCAAGCT
SDHA	Forward	TGGGAACAAGAGGGCATCTG
	Reverse	CCACCACTGCATCAAATTCATG

*MGB: minor grove binder with non fluorescent quencher; miR-199a-5p: microRNA excised from the 5' arm of microRNA-199a precursor; RNU44: small nucleolar RNA, C/D box 44; HMBS: hydroxymethyl-bilane synthase; DDR1: discoidin domain receptor-1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HPRT1: hypoxanthine phosphoribosyl-transferase 1; SDHA: succinate dehydrogenase complex, subunit A.

ISSN: 1476-4598