Figure 3
From: MiR-221 and miR-222 target PUMA to induce cell survival in glioblastoma
Expression of PUMA abrogates miR-221/222 survival function. (A) H4 cells were transfected pcDNA-PUMA (not including the 3'UTR), and PUMA expression was measured by Western blot assay. β-actin protein was regarded as endogenous normalizer. (B) H4 cells were transfected with pMSCV-miR-221/222, then treated with serum starvation and cell apoptosis was Annexin V analysis. (C) H4 cells were transfected with pcDNA-PUMA (not including the 3'UTR) and pMSCV-miR-221/222, and cell apoptosis, caspase activity and mitochondrial membrane potential were measured by Annexin V analysis, caspase 3/7 activity assay and JC-1 staining. * P < 0.05 compared with control group, ** P < 0.01 compared with control group.