GGTI-298 as a single agent ( A and B ) or combined with TRAIL ( C and D ) inhibits the growth ( A and C ) and induces apoptosis ( B and D ) of human NSCLC cells. A and C, The indicted cell lines were seeded in 96-well cell culture plates and treated the next day with the given concentrations of GGTI-298 (A) or with the given concentrations of GGTI-298 alone, TRAIL alone, and their combinations as indicated (C). After 3 days (A) or 24 h (C), cell number was estimated using the SRB assay. Data are the means of four replicate determinations; Bars, ± SDs. B and D, The indicated cell lines were treated with 15 μM GGTI-298 for 48 h (B) or with the indicated doses of TRAIL alone, 15 μM GGTI-298 alone, and their respective combinations for 24 h (D). Apoptosis were then determined by Annexin V staining.