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Figure 1 | Molecular Cancer

Figure 1

From: Sprouty1, a new target of the angiostatic agent 16K prolactin, negatively regulates angiogenesis

Figure 1

16 K hPRL treatment increases SPRY1 mRNA and protein levels in primary and human endothelial cells. A. SPRY1 mRNA expression measured by qRT-PCR in RNA extracted from ABAE cells incubated for 1 to 6 hours with 16 K hPRL (10 nM). Data were normalized to the GAPDH transcript level. B. SPRY1 protein expression analyzed by Western blotting on total protein extracted from ABAE cells treated for 1 to 6 hours with 16 K hPRL (10 nM). The level of beta-tubulin was measured as an internal control. Bottom panel: quantification of the top panel. A densitometry analysis was performed using ImageJ software and showed the SPRY1/beta tubulin intensity. C. The subcellular localization of SPRY1 was determined in ABAE cells by immunofluorescence microscopy using an antibody specific for SPRY1 (red) and a dapi staining for nuclei (blue) A, B. Mean fold change versus untreated cells (/) is reported with the corresponding SD (n = 3).*: significant at p < 0.05. The results shown are representative of at least three independent experiments.

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