Figure 3

SPRY1 expression in endothelial cells is dependent of NF-κB activity. A. Luciferase activity of ABAE cells transfected with the pElam-Luc reporter gene vector and cultured for 1 h with or without treatment with increasing concentrations of BAY 1170-82 prior to incubated for 3 h without specific treatment (control) or with 16 K hPRL (10 nM). Luciferase activities were normalized to the β-galactosidase activity. B. SPRY1 mRNA expression analyzed by qRT-PCR in RNA extracted from ABAE cells cultured with or without treatment with BAY 1170-82 (5 μM) prior to 2 h-incubation with 16 K hPRL (10 nM). Data were normalized to the GAPDH transcript level. Data are mean fold change + SD (n = 3), *: significant at p < 0.05. The results shown are representative of at least three independent experiments.