Multispecies alignment and genomic organization of miR-23a~27a~24-2 cluster. a. Alignment of the mature sequences of miR-23a, miR-27a and miR-24 among five species indicate that these miRNAs are highly conserved. b. The structure of the 2159 nucleotide pre-miR-transcript of hsa-mir-23a~27a~24-2, the structure of individual miRNAs shown is derived from the Mfold program http://mfold.bioinfo.rpi.edu/cgi-bin/rna-form1.cgi with the input of their respective precursor sequences. The promoter region of this cluster spanning -603 to +36 is well characterized; it includes a GC rich region (-530 to -410) where transcription factors are predicted to bind and the transcription start site (TSS) spans 0 to 124 region. The poly-A tail is from 1752 to 1757.