Figure 7

Effects of PKCι pseudosubstrate inhibitor peptide on cell proliferation. A. U87MG cells were plated at equal densities and 24 h later either control treated or treated with 20 or 50 μM PS-I and viable cell numbers were determined using trypan blue exclusion at days 0, 1, 2 and 3 after treatment. B. Representative examples comparing mitosis in control cells and cells treated with 20 μM PS-I. Still images from videomicroscopy are shown, with times indicated on the left. A full video is shown in Supplementary S3. C. Quantitation of the number of mitotic events observed during live cell image analysis (17 h) for U87MG cells control treated or treated with 20 μM PS-I. Data are the mean +/- SD of three independent videomicroscopy experiments for each condition. Inset table indicates the number of normal mitotic events (total starting cell numbers from the three experiments in parentheses) and the number of abnormal (delayed) mitotic events observed over the 17 h analysis for control treated and PS-I treated cells.