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Figure 1 | Molecular Cancer

Figure 1

From: ErbB2 enhances mammary tumorigenesis, oncogene-independent recurrence and metastasis in a model of IGF-IR-mediated mammary tumorigenesis

Figure 1

ErbB2 overexpression in RM11A cells did not affect activation of Akt and Erk. ErbB2 and phosphorylated ErbB2 overexpression were consistently observed by Western blotting in two independent stable clones transfected with the ErbB2 expression construct (RM11A+Dox/ErbB2) (a). ErbB2 expression was quantified after Western blotting and normalized to the loading control β-actin. Overexpression of both ErbB2 and pErbB2 was ~2-3 fold higher in both RM11A+Dox/ErbB2 clones compared to RM11A+Dox clones (b). Representative Western blots of pAkt and pErk (c). Average relative pAkt and pErk levels were determined to be unchanged in RM11A+Dox/ErbB2 cells compared to RM11A+Dox cells (d).

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