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Figure 6 | Molecular Cancer

Figure 6

From: Prognostic significance and therapeutic implications of centromere protein F expression in human nasopharyngeal carcinoma

Figure 6

FTI-277 Synergizes with cisplatin to enhance the cytotoxicity on high CENP-F expression cells. A. Dose-response curves for FTI-277. The viability of each cell line (CNE1 cell line (triangle), HONE1 cell line (black square)) was plotted against the concentration of drug used in a 72 h treatment. B-C. Shifting of dose-response curves of cisplatin by ZOL. HONE1 (B) and CNE1 (C) were assayed using MTT assay. The dose-response curves of cisplatin in the presence of ZOL (black square) or the absence of ZOL (triangle). The IC50 values are shown. D. ZOL or FTI-277 reduces CENP-F from the kinetochore during metaphase and presents misaligned chromosomes. Immunofluorescence staining of Tubulin, DNA and CENP-F in untreated and drug treated HONE1 cells. Representative mitotic cells in metaphase are shown for control and drug treated cells. Bar, 5 μm. Experiments were repeated at least three times and a representative experiment is shown.

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