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Figure 3 | Molecular Cancer

Figure 3

From: Withanolide D induces apoptosis in leukemia by targeting the activation of neutral sphingomyelinase-ceramide cascade mediated by synergistic activation of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase

Figure 3

Effect of WithaD on MAPKs in K562 and MOLT-4 cells. (A) K562 and MOLT-4 cells were treated with WithaD at the indicated time and cell lysates were resolved in SDS-PAGE (10%). Western blot analysis showed higher level of phosphorylation of JNK and p38 whereas reduced level of p-ERK was observed in different time duration. (B) MOLT-4 cells were treated with WithaD at the indicated time and p-JNK+ and p-p38+ cells were analyzed by flow cytometry. (C) K562 and MOLT-4 cells were pretreated with SP600125 (10 μM) and SB203580 (10 μM) for 1 hr, then treated with withaD for another 48 hr and % of annexin V+ cells were determined using flow cytometer. Results are the mean ± S.D. in duplicate in two independent experiments. *indicates statistically significant difference (P < 0.005) between SP600125 treated and untreated cells. **indicates statistically significant difference (P < 0.005) between SP600125 + SB203580 treated and untreated cells. # considered not significant difference (P = 0.125) between SB203580 treated and untreated cells.

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