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Figure 7 | Molecular Cancer

Figure 7

From: Withanolide D induces apoptosis in leukemia by targeting the activation of neutral sphingomyelinase-ceramide cascade mediated by synergistic activation of c-Jun N-terminal kinase and p38 mitogen-activated protein kinase

Figure 7

WithaD induced apoptosis in primary cells from both myeloid and lymphoid patients. (A) WithaD reduced the viability of the cells from pediatric myeloid, T- and B-ALL patients in 24 and 48 hr in a dose dependent manner as assessed by trypan blue dye exclusion test. (B) WithaD potentially induced apoptosis in primary cells from patients. WithaD treated primary cells from representative lymphoid and myeloid patients showed increased annexin V+/PI- (down right quadrant) and annexin V+/PI+ (upper right quadrant) cells by flow cytometry. (C) A representative SEM micrograph showing typical blebbing in cell membrane in untreated (×10000) and treated (×10000) lymphoblast. (D) Cell lysates of primary cells from patients were resolved in 10% SDS-PAGE. Western blot analysis showed the cleaved caspase 3, changes in Bcl-2 and Bax expression, after WithaD treatment in a time dependent manner. b-actin was used as loading control. (E) Cell lysates of primary cells from patients were resolved in 10% SDS-PAGE. Western blot analysis showed enhanced phosphorylation of JNK and p38 MAPK in response to WithaD treatment in a time dependent manner. β-actin was used as loading control.

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