EBNA1 induces ATF-2 and c-Myc in Ad/AH cells. (a) RT-PCR analysis shows that transcription of ATF-2 is increased in EBNA1-expressing cells. (b) Western blot analysis of total, mono- (Thr71) and dual-phosphorylated (Thr69/71) ATF-2 levels in Ad/AH-Neo and -EBNA1 cells. (c) and (d) IF staining for total ATF-2. (e) RT-PCR analysis shows that transcription of c-myc is increased in EBNA1-expressing cells. (f) Luciferase reporter assay following transient transfection of Ad/AH cells stably expressing c-MycER and either EBNA1 or an empty vector control plasmid (Neo) with a c-Myc luciferase reporter plasmid (pX-CMVp-Luc) with treatment of cells with 0 nM, 250 nM, 500 nM or 1000 nM 4-Hydroxytamoxifen for 24 hours.