Figure 6
From: Nuclear export regulation of COP1 by 14-3-3σ in response to DNA damage
14-3-3σ blocks COP1-mediated p53 nuclear export regardless of MDM2 status. (A) 14-3-3σ blocks COP1-mediated p53 nuclear export. H1299 cells were cotransfected as indicated with RFP-COP1, GFP-p53 or Flag-14-3-3σ. Live-cell images were captured using a fluorescence microscope 1 day after transfection. The bar graph shows quantification of nuclear p53 localization. Error bars represent 95% confidence intervals. Scale bar, 10 μm. (B) p53-/- MDM2-/- MEF cells were cotransfected with the indicated plasmids. Live-cell images were captured using a fluorescence microscope 1 day after transfection as described in (A). The bar graph shows quantification of nuclear p53 localization. Error bars represent 95% confidence intervals. Scale bar, 10 μm.