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Figure 10 | Molecular Cancer

Figure 10

From: Anti-angiogenic effects of differentiation-inducing factor-1 involving VEGFR-2 expression inhibition independent of the Wnt/β-catenin signaling pathway

Figure 10

Effects of Wnt3a on VEGFR-2. (A) Wnt3a suppressed VEGFR-2 expression. HUVECs were incubated with or without Wnt3a (200 ng/ml = 5.35 nM) for 24 h and the samples were subjected to Western blot analysis for VEGFR-2. The levels of protein bands were quantified and are shown as percentage of the control level at time 0. Values are mean ± SE of three independent experiments. The asterisk indicates *P < 0.05 versus control. (B) Wnt3a suppressed the VEGFR-2 promoter activity. HUVECs were co-transfected with VEGFR-2 pGL-3 or TOPflash and pRL-SV40. After 24 h incubation, cells were stimulated with or without Wnt3a (200 ng/ml) for 12 h. Luciferase activity is shown as percentages of the control. Values are mean ± SE of three independent experiments. The asterisk indicates *P < 0.05 versus control.

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Molecular Cancer

ISSN: 1476-4598