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Figure 2 | Molecular Cancer

Figure 2

From: Anti-angiogenic effects of differentiation-inducing factor-1 involving VEGFR-2 expression inhibition independent of the Wnt/β-catenin signaling pathway

Figure 2

The effect of DIF-1 on cyclin D1 protein amount. (A) Time-course. HUVECs were incubated with or without DIF-1 (30 μM) for the periods indicated. Samples were subjected to Western blot analysis using the anti-cyclin D1 antibody. The membrane was re-probed with the anti-GAPDH antibody. The levels of protein bands were quantified and shown as percentages of the control level at time 0. Values are mean ± SE of three independent experiments. The asterisk indicates *P < 0.01 and **P < 0.001 versus control. (B) Dose dependency. HUVECs were incubated with or without various amount of DIF-1 for 4 h. Samples were subjected to Western blot analysis using the anti-cyclin D1 antibody. The membrane was re-probed with the anti-GAPDH antibody. The levels of protein bands were quantified and shown as percentages of the control level. Values are mean ± SE of three independent experiments. The asterisk indicates *P < 0.001 versus control. (C) Effect of MG132. HUVECs were pretreated with MG132 (10 μM) for 3 h and incubated with or without DIF-1 (30 μM) for 6 h. Samples were subjected to Western blot analysis using the anti-cyclin D1 antibody and the membrane was re-probed with the anti-GAPDH antibody. The levels of protein bands are quantified and shown as percentages of the degraded amounts. Values are mean ± SE of three independent experiments. The asterisk indicates *P < 0.01 and versus control.

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Molecular Cancer

ISSN: 1476-4598