Skip to main content
Figure 8 | Molecular Cancer

Figure 8

From: Anti-angiogenic effects of differentiation-inducing factor-1 involving VEGFR-2 expression inhibition independent of the Wnt/β-catenin signaling pathway

Figure 8

DIF-1 reduced VEGFR-2 mRNA level and promoter activity. (A) The effects of DIF-1 on the VEGFR-2 mRNA levels. Total RNAs were extracted from HUVECs treated with or without DIF-1 (30 μM) for 24 h. The VEGFR-2 mRNA levels were determined by TaqMan quantitative real-time RT-PCR. Values are mean ± SE of four independent experiments. The asterisk indicates *P < 0.05 versus control. (B and C) The effects of DIF-1 on the human VEGFR-2 gene promoter activity on HUVECs (B) or BAECs (C). HUVECs or BAECs were co-transfected with VEGFR-2 pGL-3 and pRL-SV40. After 24 h incubation, HUVECs and BAECs were stimulated with or without DIF-1 (30 μM) for the given periods. Luciferase activity is shown as percentages of the control level at time 0. Values are mean ± SE of three independent experiments. The asterisk indicates *P < 0.01 **P < 0.001 ***P < 0.0001 versus control.

Back to article page

Molecular Cancer

ISSN: 1476-4598