HOX11 is Post-Translationally Modified During Mitosis in a Dose- and Time-Dependent Manner in Proportion to the Percentage of Mitotic Cells. (A) Immunoblot analysis of HOX11 and β-actin levels in HOX11-3T3 cultures following treatment with 100 μg/ml aphidocolin (aph), aphidocolin followed by release into fresh medium containing 10% fetal calf serum for 4 h (aph+4h), 60 μg/ml genistein (gen), 400 μg/ml colchicine (colc), 100 nM nocodazole (noc) or 3 μg/ml cytochalasin B (cytoB). Flow cytometric profiles of DNA content and percentage of cells containing a 4n complement of DNA are indicated. (B) Immunoblot analysis of HOX11 and β-actin expression in HOX11-3T3 cultures treated with varying doses of nocodazole for 17 hours (C) or with 50 nM nocodazole for varying times. Flow cytometric profiles of DNA content and percentage of cells containing a 4n complement of DNA are indicated. Densitometric analysis of post-translationally modified band intensity compared to the percentage of cells containing a 4n complement of DNA reveals a statistically significant linear relationship (R2 > 0.9). (D) Immunoblot analysis of HOX11 expression in sorted HOX11-3T3 subpopulations following Hoechst 33342 staining.