Figure 2
From: Phosphorylation of HOX11/TLX1 on Threonine-247 during mitosis modulates expression of cyclin B1
HOX11 is Phosphorylated During Mitosis. (A) HOX11 immunoprecipitates from 35S-γATP radiolabelled HOX11-3T3 cultures following treatment with nocodazole (500 ng/ml) or nocodazole supplemented with staurosporine (50 μM). (B) Immunoblots of HOX11 and β-actin protein levels in total and phosphoenriched fraction of nocodazole-treated lysates. (C) Immunoblot of HOX11 protein levels of untreated and nocodazole-treated HOX11 lysates following incubation with various levels of alkaline phosphatase. Ponceau S staining demonstrating exogenous addition of alkaline phosphatase to the in vitro enzymatic reaction. (D) Immunoblot of HOX11 protein levels following treatment with nocodazole and 0.05, 0.5, 5.0 or 50 μM staurosporine. (E) Immunoblot of HOX11 protein levels following treatment with nocodazole in media supplemented with 0-4 U of recombinant protein phosphatase 1 catalytic subunit (rPP1cs).