Figure 4
Effect of ERCC1-XPF over-expression on ICL repair and cisplatin sensitivity. A) 833 K cells were transfected with plasmid pEF6(XPF-IRES-ERCC1). Immunoblot analysis of XPF and ERCC1 proteins in extracts harvested at the indicated time points. RPA2 served as loading control. B) 833 K cells (non-transfected, transfected with pEF6(ERCC1-IRES-XPF), transfected with vector pEF6, mock transfected) were treated with cisplatin (15 μg/ml) for 1 h, the level of interstrand crosslinking was determined 7 and 24 h post-treatment. Each column represents the mean of 3 to 6 replicate experiments. C) Cells were transfected with plasmid pEF6(XPF-IRES-ERCC1) for 24 h, treated with cisplatin (12 and 15 μg/ml) for 1 h and post-incubated for 96 h. Apoptosis was measured by flow cytometry (sub-G1 content). * represents statistical significance.